Dark Hydrogen Fermentation From Paper Mill Effluent (PME): The influence of Substrate Concentration and Hydrolysis

Document Type: Research Article

Authors

Department of Chemical Engineering, Isfahan University of Technology, Isfahan, Iran

Abstract

Paper mill effluent (PME) was used as an organic feedstock for production of biohydrogen via dark fermentation using heat-shock pretreated anaerobic sludge under mesophilic conditions. The influence of substrate concentration (5, 10 and 15 g-COD/L) and the initial pH (5 and 7) on the efficiency of dark hydrogen fermentation from PME were investigated. The highest hydrogen yield of 55.4 mL/g-COD was obtained at substrate concentration and pH of 5 g-COD/L and 5, respectively. By increasing the concentration of substrate from 5 to 10 and 15 g-COD/L, at fixed initial pH, the hydrogen production efficiency was reduced from 55.4 mL/g-COD to 38.5 and 32.7 mL/g-COD. Furthermore, by increasing pH from 5 to 7, biohydrogen efficiency was reduced up to 40.8%. Different hydrolysis of PME including acidic, acidic-thermal and alkaline hydrolysis prior to fermentation were studied which the alkaline hydrolysis led to the highest hydrogen yield of 62.2 mL/g-COD. Moreover, methane production efficiency of 569 mL/g-COD was obtained at substrate concentration and pH of 5 g-COD/L and 7, respectively.
 
Paper mill effluent (PME) was used as an organic feedstock for production of biohydrogen via dark fermentation using heat-shock pretreated anaerobic sludge under mesophilic conditions. The influence of substrate concentration (5, 10 and 15 g-COD/L) and the initial pH (5 and 7) on the efficiency of dark hydrogen fermentation from PME were investigated. The highest hydrogen yield of 55.4 mL/g-COD was obtained at substrate concentration and pH of 5 g-COD/L and 5, respectively. By increasing the concentration of substrate from 5 to 10 and 15 g-COD/L, at fixed initial pH, the hydrogen production efficiency was reduced from 55.4 mL/g-COD to 38.5 and 32.7 mL/g-COD. Furthermore, by increasing pH from 5 to 7, biohydrogen efficiency was reduced up to 40.8%. Different hydrolysis of PME including acidic, acidic-thermal and alkaline hydrolysis prior to fermentation were studied which the alkaline hydrolysis led to the highest hydrogen yield of 62.2 mL/g-COD. Moreover, methane production efficiency of 569 mL/g-COD was obtained at substrate concentration and pH of 5 g-COD/L and 7, respectively.

Keywords


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